Hohenheim Research Center for One Health Sciences

Struktur-Funktions-Beziehungen

 Projekte
  • Erhöhte Phagensicherheit in Molkereien durch hochspezifische molekulare Phagen-Nachweissysteme und eine orthogonale Prozessstrategie zur Phagenreduktion in Molke (2017-2019), mehr
  • Assembly of the phage T7 DNA-translocator complex (Kuhn, Nagler, Lupo) (2013-2016), mehr
  • Capsid assembly des Bakteriophagen T4: Destination der Membranlokalisation des T4-Phagenkopfes beim Assemblyprozess (Kuhn, Lupo, Nagler) (2010-2016), mehr
  • Struktur und Funktion der Membraninsertase YidC: Der molekulare Mechanismus der Substraterkennung und die Molekülstruktur von YidC wird anhand des M13 Procoat Proteins analysiert (Kuhn, Spann, Teßmer, Kiefer) (2009-2016), mehr
  • Zelluläre Lokalisation des Portalproteins vom Bakteriophagen T4 (Lupo, Kuhn)
  • Membraneinbau und Orientierung des Phagen Coat Proteins Pf3 in Liposomen
Publikationen
  • Wenzel, Sabrina; Shneider, Mikhail M.; Leiman, Petr G.; Kuhn, Andreas; Kiefer, Dorothee (2020): The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of Escherichia coli. In: Viruses 12 (10).DOI: 10.3390/v12101135.
  • Loh, Belinda; Kuhn, Andreas; Leptihn, Sebastian (2018): The fascinating biology behind phage display. Filamentous phage assembly. In: Molecular microbiology. DOI: 10.1111/mmi.14187.
  • Kiefer, Dorothee; Kuhn, Andreas (2018): YidC-mediated membrane insertion. In: FEMS Microbiology Letters 365 (12). DOI: 10.1093/femsle/fny106.
  • Serdiuk, Tetiana; Steudle, Anja; Mari, Stefania A.; Manioglu, Selen; Kaback, H. Ronald; Kuhn, Andreas; Müller, Daniel J. (2019): Insertion and folding pathways of single membrane proteins guided by translocases and insertases. In: Science advances 5 (1), eaau6824. DOI: 10.1126/sciadv.aau6824.
  • Kiefer D, Kuhn A. YidC-mediated membrane insertion. FEMS Microbiol Lett. 2018 Jun 1;365(12). doi: 10.1093/femsle/fny106.
  • Spann D, Pross E.; Chen Y.; Dalbey RE.; Kuhn A. (2018): Each protomer of a dimeric YidC functions as a single membrane insertase. Bakteriophage 589 (8). doi:10.1038/s41598-017-18830-9.
  • Kuhn A, Kiefer D (2017): Membrane protein insertase YidC in bacteria and archaea. Molecular Microbiology 103 (4), 590–594. doi:10.1111/mmi.13586.
  • Loh, B.; Haase, M.; Mueller, L.; Kuhn, A.; Leptihn, S. (2017): The transmembrane morphogenesis protein gp1 of filamentous phages contains walker A and walker B motifs essential for phage assembly. In: Viruses 9 (4). DOI: 10.3390/v9040073.
  • Leptihn S, Gottschalk J, Kuhn A (2016): T7 ejectosome assembly: A story unfolds. Bacteriophage 6 (1), e1128513. doi:10.1080/21597081.2015.1128513.
  • Lupo D, Leptihn S, Nagler G, Haase M, Molineux I, Kuhn A (2015): The T7 ejection nanomachine components gp15-gp16 form a spiral ring complex that binds DNA and a lipid membrane. Virology 486, 263–71. doi:10.1016/j.virol.2015.09.022.
  • Chen Y, Soman R, Shanmugam SK, Kuhn A, Dalbey RE (2014): The role of the strictly conserved positively charged residue differs among the Gram-positive, Gram-negative, and chloroplast YidC homologs. The Journal of Biological Chemistry 289, 35656–67. doi:10.1074/jbc.M114.595082.
  • Dalbey RE, Kuhn A (2014): How YidC inserts and folds proteins across a membrane. Nature Structural and Molecular Biology 21, 435–6. doi:10.1038/nsmb.2823.
  • Dalbey RE, Kuhn A, Zhu L, Kiefer D (2014): The membrane insertase YidC. BBA – Molecular Cell Research 1843 (8), 1489–1496. DOI: 10.1016/j.bbamcr.2013.12.022.
  • Ganz, Holly H.; Law, C.; Schmuki, M.; Eichenseher, F.; Calendar, R.; Loessner, M. J. et al. (2014): Novel giant siphovirus from Bacillus anthracis features unusual genome characteristics. In: PLoS ONE 9 (1). DOI: 10.1371/journal.pone.0085972.
  • Kuhn A (2014): Introduction to special issue on protein trafficking and secretion. BBA – Molecular Cell Research 1843 (8), 1429–32. doi:10.1016/j.bbamcr.2014.04.001.
  • Seitl I, Wickles S, Beckmann R, Kuhn A, Kiefer D (2014):The C-terminal regions of YidC from Rhodopirellula baltica and Oceanicaulis alexandrii bind to ribosomes and partially substitute for SRP receptor function in Escherichia coli. Molecular Microbiology 91, 408–21. doi:10.1111/mmi.12465.
  • Soman R, Yuan J, Kuhn A, Dalbey RE (2014): Polarity and charge of the periplasmic loop determine the YidC and sec translocase requirement for the M13 procoat lep protein. Journal of Biological Chemistry 289, 1023–32. doi:10.1074/jbc.M113.522250.
  • Winterfeld S, Ernst S, Borsch M, Gerken U, Kuhn A (2013): Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC. PLoS ONE 8, e59023. doi:10.1371/journal.pone.0059023.
  • Dalbey RE, Kuhn A (2012): Protein traffic in Gram-negative bacteria – how exported and secreted proteins find their way. FEMS Microbiology Review 36, 1023–45. doi:10.1111/j.1574-6976.2012.00327.x.
  • Klenner C, Kuhn A (2012): Dynamic disulfide scanning of the membrane-inserting Pf3 coat protein reveals multiple YidC substrate contacts. Journal of Biological Chemistry 287, 3769–76. doi:10.1074/jbc.M111.307223.
  • Neugebauer SA, Baulig A, Kuhn A, Facey SJ (2012): Membrane protein insertion of variant MscL proteins occurs at YidC and SecYEG of Escherichia coli. Journal of Molecular Biology 417, 375–86. doi:10.1016/j.jmb.2012.01.046.
  • Ploß M (2012): Biogenese und Virusassembly des filamentösen Coliphagen M13 2012.
  • Quinten TA, Kuhn A (2012): Membrane interaction of the portal protein gp20 of bacteriophage T4. Journal of Virology 86, 11107–14. doi:10.1128/JVI.01284-12.
  • Zhu L, Klenner C, Kuhn A, Dalbey RE (2012): Both YidC and SecYEG are required for translocation of the periplasmic loops 1 and 2 of the multispanning membrane protein TatC. Journal of Molecular Biology 424, 354–67. doi:10.1016/j.jmb.2012.09.026.
  • Ernst S, Schonbauer A-K, Bar G, Borsch M, Kuhn A (2011): YidC-driven membrane insertion of single fluorescent Pf3 coat proteins. Journal of Molecular Biology 412 (2), 165–175. DOI: 10.1016/j.jmb.2011.07.023.
  • Ploss M, Kuhn A (2011): Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage. BMC Microbiology 11, 211. doi:10.1186/1471-2180-11-211.
  • Stiegler N (2011): Membraninsertion des Phagenproteins M13 procoat in Lipidvesikel mit rekonstituiertem Escherichia coli Yid. 2011
  • Stiegler N, Dalbey RE, Kuhn A (2011): M13 procoat protein insertion into YidC and SecYEG proteoliposomes and liposomes. Journal of Molecular Biology 406 (3), 362–370. DOI: 10.1016/j.jmb.2010.12.036.
  • Ploss M, Kuhn A (2010): Kinetics of filamentous phage assembly. Physical biology 7 (4), 45002. DOI: 10.1088/1478-3975/7/4/045002.
  • Nagler C, Nagler G, Kuhn A, (2007): Cysteine residues in the transmembrane regions of M13 procoat protein suggest that oligomeric coat proteins assemble onto phage progeny. Journal of Bacteriology 189 (7), 2897–2905. DOI: 10.1128/JB.01551-06.